[The relationship between GAPDH mRNA degradation in the mouse liver and postmortem interval].

[The relationship between GAPDH mRNA degradation in the mouse liver and postmortem interval].

Fa Yi Xue Za Zhi. 2010 Dec;26(6):425-7

Authors: Wu HY, Wang KJ, Zhang L, Guo LW, Guo JN, Fan AY

Abstract
OBJECTIVE: To explore the relationship between degradation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the mouse liver and postmortem interval (PMI).
METHODS: Sixty NIH mice were sacrificed by cervical dislocation and suffocation, and then placed into 10 degrees C and 25 degrees C temperature-controlling systems. The changes of GAPDH mRNA in the liver were detected by two-step fluorimetric reverse transcriptase polymerase chain reaction (RT-PCR) technique and nucleic acids protein cryoscope from 0 to 48 h postmortem.
RESULTS: In the mouse liver, the amplification products of GAPDH mRNA could be examined within 48 h postmortem in 10 degrees C temperature-controlling system and within 36 h postmortem in 25 degrees C temperature-controlling system. The amplification products showed a decreasing tendency.
CONCLUSION: Degradation of GAPDH mRNA in the mouse liver is negative correlation with PMI. GAPDH mRNA could be a new marker for estimation of PMI.

PMID: 21425603 [PubMed - indexed for MEDLINE]

[Estimation of postmortem interval using microRNA and 18S rRNA degradation in rat cardiac muscle].

[Estimation of postmortem interval using microRNA and 18S rRNA degradation in rat cardiac muscle].

Fa Yi Xue Za Zhi. 2010 Dec;26(6):413-7

Authors: Li WC, Ma KJ, Zhang P, Wang HJ, Shen YW, Zhou YQ, Zhao ZQ, Ma D, Chen L

Abstract
OBJECTIVE: To explore the relationship between the time-dependent level changes of microRNA and 18S rRNA and the different postmortem interval (PMI) in rat cardiac muscle.
METHODS: SD rats were sacrificed by cervical dislocation and placed at ambient temperature 25 degrees C with a humidity of 50%. Total RNA was extracted from the rat cardiac muscle at different time points after death. The levels of miR-1-2 and 18S rRNA were examined using real-time PCR in rat cardiac muscle. The results were expressed by cycle threshold (Ct) value to explore relationship between PMI and Ct value, and the regression functions were established to estimate PMI.
RESULTS: The miR-1-2 level in rat myocardial tissue showed no significant changes within 120 h after death, and then began to decline. The 18S rRNA level increased gradually within 96 h after death, and then declined slowly. The nonlinear relationships were established between Ct value (18S rRNA), deltaCt value (difference between 18S rRNA and miR-1-2) and PMI. The R2 of conics fitting were 0.9487 and 0.8072, respectively.
CONCLUSION: Ct value of 18S rRNA and deltaCt value present a good correlation with PMI, and can be markers for estimating early PMI.

PMID: 21425600 [PubMed - indexed for MEDLINE]

[Estimation of postmortem interval using microRNA and 18S rRNA degradation in rat cardiac muscle].

[Estimation of postmortem interval using microRNA and 18S rRNA degradation in rat cardiac muscle].

Fa Yi Xue Za Zhi. 2010 Dec;26(6):413-7

Authors: Li WC, Ma KJ, Zhang P, Wang HJ, Shen YW, Zhou YQ, Zhao ZQ, Ma D, Chen L

Abstract
OBJECTIVE: To explore the relationship between the time-dependent level changes of microRNA and 18S rRNA and the different postmortem interval (PMI) in rat cardiac muscle.
METHODS: SD rats were sacrificed by cervical dislocation and placed at ambient temperature 25 degrees C with a humidity of 50%. Total RNA was extracted from the rat cardiac muscle at different time points after death. The levels of miR-1-2 and 18S rRNA were examined using real-time PCR in rat cardiac muscle. The results were expressed by cycle threshold (Ct) value to explore relationship between PMI and Ct value, and the regression functions were established to estimate PMI.
RESULTS: The miR-1-2 level in rat myocardial tissue showed no significant changes within 120 h after death, and then began to decline. The 18S rRNA level increased gradually within 96 h after death, and then declined slowly. The nonlinear relationships were established between Ct value (18S rRNA), deltaCt value (difference between 18S rRNA and miR-1-2) and PMI. The R2 of conics fitting were 0.9487 and 0.8072, respectively.
CONCLUSION: Ct value of 18S rRNA and deltaCt value present a good correlation with PMI, and can be markers for estimating early PMI.

PMID: 21425600 [PubMed - indexed for MEDLINE]

[The relationship between GAPDH mRNA degradation in the mouse liver and postmortem interval].

[The relationship between GAPDH mRNA degradation in the mouse liver and postmortem interval].

Fa Yi Xue Za Zhi. 2010 Dec;26(6):425-7

Authors: Wu HY, Wang KJ, Zhang L, Guo LW, Guo JN, Fan AY

Abstract
OBJECTIVE: To explore the relationship between degradation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the mouse liver and postmortem interval (PMI).
METHODS: Sixty NIH mice were sacrificed by cervical dislocation and suffocation, and then placed into 10 degrees C and 25 degrees C temperature-controlling systems. The changes of GAPDH mRNA in the liver were detected by two-step fluorimetric reverse transcriptase polymerase chain reaction (RT-PCR) technique and nucleic acids protein cryoscope from 0 to 48 h postmortem.
RESULTS: In the mouse liver, the amplification products of GAPDH mRNA could be examined within 48 h postmortem in 10 degrees C temperature-controlling system and within 36 h postmortem in 25 degrees C temperature-controlling system. The amplification products showed a decreasing tendency.
CONCLUSION: Degradation of GAPDH mRNA in the mouse liver is negative correlation with PMI. GAPDH mRNA could be a new marker for estimation of PMI.

PMID: 21425603 [PubMed - indexed for MEDLINE]

Database of post-mortem rectal cooling cases under strictly controlled conditions: a useful tool in death time estimation.

Database of post-mortem rectal cooling cases under strictly controlled conditions: a useful tool in death time estimation.

Int J Legal Med. 2012 Jan;126(1):79-87

Authors: Muggenthaler H, Sinicina I, Hubig M, Mall G

Abstract
The most common method used in determining the estimated time since death in the early post-mortem phase is back-calculation based on rectal temperature decrease. Cooling experiments are essential for model generation and validation. Post-mortem temperature models are necessary to perform back-calculations. Thus far, cooling experiments have not been performed under controlled environmental conditions. The present study provides data on 84 post-mortem cooling experiments under strictly controlled environmental conditions. For a period of 5 years, starting in 2003, deceased persons with a known time of death and known environmental conditions at the death scene were transferred to a climatic chamber for the process of body cooling. The environmental temperature was programmed to the death scene temperature and kept constant throughout the process of body cooling. Rectal and ambient temperatures were measured every minute. Relevant case-specific information was summarized in a FileMaker database. The database serves as a reference tool for the comparison of real cases in forensic routine and to check the plausibility of model-derived estimates.

PMID: 21538225 [PubMed - indexed for MEDLINE]

Database of post-mortem rectal cooling cases under strictly controlled conditions: a useful tool in death time estimation.

Database of post-mortem rectal cooling cases under strictly controlled conditions: a useful tool in death time estimation.

Int J Legal Med. 2012 Jan;126(1):79-87

Authors: Muggenthaler H, Sinicina I, Hubig M, Mall G

Abstract
The most common method used in determining the estimated time since death in the early post-mortem phase is back-calculation based on rectal temperature decrease. Cooling experiments are essential for model generation and validation. Post-mortem temperature models are necessary to perform back-calculations. Thus far, cooling experiments have not been performed under controlled environmental conditions. The present study provides data on 84 post-mortem cooling experiments under strictly controlled environmental conditions. For a period of 5 years, starting in 2003, deceased persons with a known time of death and known environmental conditions at the death scene were transferred to a climatic chamber for the process of body cooling. The environmental temperature was programmed to the death scene temperature and kept constant throughout the process of body cooling. Rectal and ambient temperatures were measured every minute. Relevant case-specific information was summarized in a FileMaker database. The database serves as a reference tool for the comparison of real cases in forensic routine and to check the plausibility of model-derived estimates.

PMID: 21538225 [PubMed - indexed for MEDLINE]

A comparison of three established age estimation methods on an adult Spanish sample.

A comparison of three established age estimation methods on an adult Spanish sample.

Int J Legal Med. 2012 Jan;126(1):145-55

Authors: Rissech C, Wilson J, Winburn AP, Turbón D, Steadman D

Abstract
Most current methods for adult skeletal age-at-death estimation are based on American samples comprising individuals of European and African ancestry. Our limited understanding of population variability hampers our efforts to apply these techniques to various skeletal populations around the world, especially in global forensic contexts. Further, documented skeletal samples are rare, limiting our ability to test our techniques. The objective of this paper is to test three pelvic macroscopic methods [(1) Suchey-Brooks; (2) Lovejoy; and (3) Buckberry and Chamberlain] on a documented modern Spanish sample. These methods were selected because they are popular among Spanish anthropologists and because they never have been tested in a Spanish sample. The study sample consists of 80 individuals (55 males and 25 females) of known sex and age from the Valladolid collection. Results indicate that in all three methods, levels of bias and inaccuracy increase with age. The Lovejoy method performs poorly (27%) compared with Suchey-Brooks (71%) and Buckberry and Chamberlain (86%). However, the levels of correlation between phases and chronological ages are low and comparable in the three methods (<0.395). The apparent accuracy of the Suchey-Brooks and Buckberry and Chamberlain methods is largely based on the broad width of the methods' estimated intervals. This study suggests that before systematic application of these three methodologies in Spanish populations, further statistical modeling and research into the covariance of chronological age with morphological change are necessary. Future methods should be developed specific to various world populations and should allow for both precision and flexibility in age estimation.

PMID: 21656296 [PubMed - indexed for MEDLINE]

A comparison of three established age estimation methods on an adult Spanish sample.

A comparison of three established age estimation methods on an adult Spanish sample.

Int J Legal Med. 2012 Jan;126(1):145-55

Authors: Rissech C, Wilson J, Winburn AP, Turbón D, Steadman D

Abstract
Most current methods for adult skeletal age-at-death estimation are based on American samples comprising individuals of European and African ancestry. Our limited understanding of population variability hampers our efforts to apply these techniques to various skeletal populations around the world, especially in global forensic contexts. Further, documented skeletal samples are rare, limiting our ability to test our techniques. The objective of this paper is to test three pelvic macroscopic methods [(1) Suchey-Brooks; (2) Lovejoy; and (3) Buckberry and Chamberlain] on a documented modern Spanish sample. These methods were selected because they are popular among Spanish anthropologists and because they never have been tested in a Spanish sample. The study sample consists of 80 individuals (55 males and 25 females) of known sex and age from the Valladolid collection. Results indicate that in all three methods, levels of bias and inaccuracy increase with age. The Lovejoy method performs poorly (27%) compared with Suchey-Brooks (71%) and Buckberry and Chamberlain (86%). However, the levels of correlation between phases and chronological ages are low and comparable in the three methods (<0.395). The apparent accuracy of the Suchey-Brooks and Buckberry and Chamberlain methods is largely based on the broad width of the methods' estimated intervals. This study suggests that before systematic application of these three methodologies in Spanish populations, further statistical modeling and research into the covariance of chronological age with morphological change are necessary. Future methods should be developed specific to various world populations and should allow for both precision and flexibility in age estimation.

PMID: 21656296 [PubMed - indexed for MEDLINE]

Timing and sequence of emergence of permanent teeth in the Jordanian population.

Timing and sequence of emergence of permanent teeth in the Jordanian population.

Arch Oral Biol. 2012 Feb;57(2):122-30

Authors: Shaweesh AI

Abstract
OBJECTIVES: Population-specific standards of tooth emergence are essential resources for various fields of clinical and forensic dentistry. To date, such standards have not been provided for the Jordanian population. This study aimed at providing the timing and sequence of emergence of the permanent dentition in the Jordanian population.
METHODS AND RESULTS: Using multistage clustered sampling, 1240 males and 1432 females aged 4-16 years from kindergartens and primary schools in the northern, middle and southern regions of Jordan were examined for emergence of permanent teeth. The subjects were classified into 1-year age groups. For a given tooth, "present" teeth were counted and expressed as a frequency relative to the total of subjects within a given age group. Using Probit regression (SPSS version 16), the median emergence age per tooth was calculated for the total sample and for both genders. No statistically significant differences were detected between sides. In addition, mandibular teeth were generally found to emerge earlier than the corresponding maxillary teeth. However, the difference in mandibular precedence was not statistically significant in all corresponding inter-maxillary tooth pairs. Furthermore, permanent teeth were found to emerge sooner in females than in males although the inter-gender differences were non-statistically significant for first molars and central incisors.
CONCLUSIONS: The first standards of timing and sequence of permanent tooth emergence specific to the Jordanian population were provided and found to be consistent with those of Caucasian populations. These standards aid managing patients in paediatric dentistry, planning orthodontic therapy and forensic age estimation.

PMID: 21924705 [PubMed - indexed for MEDLINE]

Estimation of sex from hand and handprint dimensions in a Western Australian population.

Estimation of sex from hand and handprint dimensions in a Western Australian population.

Forensic Sci Int. 2012 May 10;

Authors: Ishak NI, Hemy N, Franklin D

Abstract
A number of recent studies have demonstrated that sex can be estimated with a high degree of expected accuracy through the analysis of anthropometric measurements of the hand. Presently, however, the majority of previous related research has been focused on a limited range of global populations. The aim of the present study, therefore, is to evaluate the accuracy of using anthropometric hand measurements for the estimation of sex in a contemporary adult Western Australian population; we also assess if sex can be accurately estimated from the measurement of handprints. The study sample comprises a total of 91 male and 110 female individuals; documented mean age for the males is 38years (range 19-68) and for the female sample it is 36years (range 18-63). A total of six linear measurements are taken from each hand and its corresponding print. Measurement data is analysed using basic univariate statistics and a series of direct and stepwise discriminant function analyses are performed to assess the sex classification potential of the hand and handprint variables. All six hand and handprint measurements are sexually dimorphic and sex explains 28.4-61.7% of the sample variance. The breadth and length of the hand contribute most significantly to sex discrimination; cross-validated sex classification accuracies range between 82.6 and 96.5% with a sex bias of ≤5%. We conclude that anthropometric measurements of the hand and handprint can be used to classify sex with a high degree of expected accuracy in a Western Australian population.

PMID: 22578812 [PubMed - as supplied by publisher]